“A Modern View of Stem Cells: Integration of Master Regulators at Multiple Levels,” will be presented by Dr. Angie Rizzino, University of Nebraska Medical Center. Efforts to identify the molecular mechanisms that control the self-renewal and pluripotency of embryonic stem cells (ESC) have led to the identification of a growing list of master regulators, including the transcription factors Sox2 and Oct4. To understand the action of these transcription factors, we have manipulated their expression in ESC and determined that small changes in master regulators dramatically alters the fate of ESC. To more fully understand how small changes in master regulators regulate the behavior of ESC, we have conducted unbiased proteomic screens to define the Sox2-interactome and determine the placement of Sox2 in the nuclear protein-protein interaction landscape of ESC. Additionally, we are studying how the expression and function of transcription factors are controlled by key signal events. Together, our studies argue that master regulators function by directly coordinating all major functions of the cells, including signal transduction, transcription, replication and DNA repair.
The seminar begins at 4:00 p.m., preceded by a reception at 3:30 p.m. The seminar is free and open to the public. The Beadle Center is located at 1901 Vine Street. The complete schedule of seminars may be found at http://biotech.unl.edu/
More details at: http://go.unl.edu/df7