“Regulation and kinetics of oxidative proline metabolism,” will be presented by Dr. Don Becker, University of Nebraska-Lincoln. The proline metabolic pathway is of importance for understanding mechanisms of bioenergetics and stress response in various organisms. The interconversion of proline and glutamate form the two main branches of the proline metabolic pathway. Proline is oxidized by proline dehydrogenase (PRODH) and ∆1-pyrroline-5-carboxylate (P5C) dehydrogenase (P5CDH) to form glutamate while P5C synthetase and P5C reductase catalyze the reduction of glutamate back to proline. To gain insights into the bioenergetics of proline metabolism and how proline affords stress protection, our laboratory is investigating the regulation and kinetics of proline oxidative metabolism. Our studies primarily focus on the proline utilization A (PutA) protein, which is a multifunctional enzyme from bacteria with PRODH, P5CDH, and DNA binding activities. Data supporting a redox mechanism for regulating the different functions of PutA will be discussed along with mechanisms of substrate channeling in proline metabolism.
The seminar begins at 4:00 p.m., preceded by a reception at 3:30 p.m. The seminar is free and open to the public. The Beadle Center is located at 1901 Vine Street. The complete schedule of seminars may be found at http://biotech.unl.edu/
More details at: http://go.unl.edu/df7